Part:BBa_K1053104:Design

FB-linker-PP7

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 356
  Illegal BglII site found at 612
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The synthesized two-part sequence for FB-linker-PP7 was ligated by overlap PCR.

Source

We used the synthesized sequences of split GFP fused with MS2 and PP7 (FA-linker-MS2, FB-linker-PP7) respectively, reference of BioBrick part BBa_K738004 (FA-linker-MS2) and BBa_K738005 (FB-linker-PP7) (ZJU-China 2012).